Bacillus subtilis division protein DivIVA - screen for stable oligomer state conditions

Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1542-3. doi: 10.1107/s0907444902014336. Epub 2002 Sep 26.

Abstract

The cell division protein DivIVA is predicted to be a coiled-coil, tropomyosin-like protein, that self-associates both in vivo and in vitro into oligomers of up to 10-12 monomers. A simple and quick screen for conditions supporting the stable oligomer structure has been developed revealing that DivIVA forms a homogeneous oligomer in the presence of PEGs (PEG 4 K or PEG 8K and PEG 1K).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / chemistry*
  • Bacterial Proteins / chemistry*
  • Cell Cycle Proteins / chemistry*
  • Crystallization
  • Electrophoresis, Polyacrylamide Gel
  • Protein Structure, Quaternary
  • Recombinant Proteins / chemistry

Substances

  • Bacterial Proteins
  • Cell Cycle Proteins
  • DivIVA protein, bacteria
  • Recombinant Proteins