Abstract
In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Atropa belladonna / drug effects
-
Atropa belladonna / enzymology*
-
Atropa belladonna / genetics
-
Blotting, Northern
-
Cell Surface Extensions / drug effects
-
Cell Surface Extensions / enzymology
-
Cells, Cultured
-
Cloning, Molecular
-
DNA, Complementary / chemistry
-
DNA, Complementary / genetics
-
Escherichia coli / genetics
-
Gene Expression Regulation, Enzymologic / drug effects
-
Gene Expression Regulation, Plant / drug effects
-
Methyltransferases / genetics*
-
Methyltransferases / metabolism
-
Molecular Sequence Data
-
Plant Proteins / genetics
-
Plant Proteins / metabolism
-
Plant Roots / drug effects
-
Plant Roots / enzymology*
-
Plant Roots / genetics
-
RNA, Messenger / drug effects
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Salicylic Acid / metabolism
-
Salicylic Acid / pharmacology*
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
Substances
-
DNA, Complementary
-
Plant Proteins
-
RNA, Messenger
-
Methyltransferases
-
salicylic acid carboxyl methyltransferase
-
Salicylic Acid