Abstract
To investigate the immune response to anti-rabies vaccination in the principal recipient (the domestic dog), four truncated fragments of the rabies virus glycoprotein were expressed as glutathione S-transferase fusion proteins. Immune sera from vaccinated rabbits and dogs were then used to probe for reactivity with these expressed proteins. In two rabbits and four dogs tested, the dominant antibody response to non-conformational antigenic sites appeared to be directed to a region of the glycoprotein between amino acids 222 and 332. The N-terminal fragment of the glycoprotein was also significantly antigenic. Further studies to assess whether the antibody response to the internal domain could neutralize the rabies Challenge Virus Standard (CVS) strain, using antibody depletion, suggested that this fraction did contribute to the ability of post-vaccination sera to neutralize and therefore protect against infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Antibodies, Viral / immunology
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Antigens, Viral / genetics
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Antigens, Viral / immunology*
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Binding Sites
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Dogs
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / immunology*
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Gene Expression
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Glycoproteins / genetics
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Glycoproteins / immunology*
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Immunodominant Epitopes / genetics
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Immunodominant Epitopes / immunology*
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Neutralization Tests
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Rabbits
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Rabies Vaccines / genetics
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Rabies Vaccines / immunology*
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Rabies virus / immunology*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Vaccination
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology*
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Viral Proteins / genetics
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Viral Proteins / immunology*
Substances
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Antibodies, Viral
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Antigens, Viral
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Epitopes, B-Lymphocyte
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Glycoproteins
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Immunodominant Epitopes
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Rabies Vaccines
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Viral Proteins