Abstract
The 3' ends of the genes for the C-terminal region of C5a peptidase from 15 Streptococcus pyogenes isolates were analyzed by PCR. Amplicons were found to differ in size. DNA sequence analysis revealed that the differences between PCR fragment sizes accorded with the number of R repeats in the C5a peptidase gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial*
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Amino Acid Sequence
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Endopeptidases / chemistry*
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Endopeptidases / genetics*
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Genetic Variation*
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Ribotyping
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Sequence Analysis, DNA
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Streptococcal Infections / microbiology
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Streptococcus pyogenes / enzymology*
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Streptococcus pyogenes / genetics*
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Streptococcus pyogenes / isolation & purification
Substances
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Adhesins, Bacterial
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DNA, Bacterial
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RNA, Ribosomal, 16S
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Endopeptidases
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C5a peptidase