FT-IR spectroscopy was applied to investigate 15 different carotenoids. The following compounds were examined: beta-carotenone (1); semi-beta-carotenon-epoxide (2); beta-apo-8'-carotenal (3); ethyl-beta-apo-8'-carotenoate (4); beta-citraurin (5); 5,6-Epoxy-beta-caroten-8'-al (6); beta-citraurin-epoxide (7); apo-10'-violaxanthal (8); persicaxanthin (9); capsylaldehyde (10); capsanthylal (11); retinol (12); retinal (13); retinoic acid (14); and bixin (15). Some characteristic functional groups (Cz.dbnd;C, Cz.dbnd;O, CHO, OH, etc.) were identified. We focused on the influence of conjugation of the keto-, aldehyde- and ester groups on the absorption of the Cz.dbnd;C bonds. This method is useful in the fast analysis of the biologically important carotenoids especially if there are small samples available.