Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies

Jason Jon Fritz; Alfred Lewin; William Hauswirth; Anupam Agarwal; Maria Grant; Lynn Shaw

doi:10.1016/s1046-2023(02)00233-5

Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies

Methods. 2002 Oct;28(2):276-85. doi: 10.1016/s1046-2023(02)00233-5.

Authors

Jason Jon Fritz¹, Alfred Lewin, William Hauswirth, Anupam Agarwal, Maria Grant, Lynn Shaw

Affiliation

¹ Department of Molecular Genetics and Microbiology, University of Florida, College of Medicine, Box 100267, Gainesville, FL 32610, USA.

PMID: 12413427
DOI: 10.1016/s1046-2023(02)00233-5

Abstract

Hammerhead ribozymes are catalytic RNAs that are being used to inhibit endogenous gene expression to study key components of basic biochemical pathways such as angiogenesis. In addition, these ribozymes have the potential to be used as components of gene therapy protocols for the treatment of disease states. We detail here a set of protocols for the design and testing of hammerhead ribozymes that will efficiently inhibit gene expression both in cell culture and in vivo.

MeSH terms

Adenoviridae / genetics
Calibration
Cells, Cultured
Endothelium / cytology
Gene Expression Regulation
Genetic Therapy / methods*
Genetic Vectors / genetics
Humans
In Vitro Techniques
Nucleic Acid Conformation
Plasmids / genetics
RNA, Catalytic / chemistry
RNA, Catalytic / genetics*
Transfection / methods*

Substances

RNA, Catalytic
hammerhead ribozyme