Abstract
Attachment of Norwalk (NV), Snow Mountain (SMV), and Hawaii (HV) virus-like particles (VLPs) to specific ABH histo-blood group antigens was investigated by using human saliva and synthetic biotinylated carbohydrates. The three distinct Norwalk-like viruses (NLVs) have various capacities for binding ABH histo-blood group antigens, suggesting that different mechanisms for NLV attachment likely exist. Importantly, antisera from NV-infected human volunteers, as well as from mice inoculated with packaged Venezuelan equine encephalitis virus replicons expressing NV VLPs, blocked the ability of NV VLPs to bind synthetic H type 1, Le(b), and H type 3, suggesting a potential mechanism for antibody-mediated neutralization of NV.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ABO Blood-Group System / metabolism*
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Animals
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Antibodies, Viral / metabolism*
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Binding Sites
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Caliciviridae Infections / immunology
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Caliciviridae Infections / prevention & control
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Caliciviridae Infections / virology
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Cell Line
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Cricetinae
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Encephalitis Virus, Venezuelan Equine / genetics
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Humans
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In Vitro Techniques
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Mice
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Neutralization Tests
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Norovirus / genetics
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Norovirus / immunology*
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Norovirus / pathogenicity
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Norwalk virus / genetics
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Norwalk virus / immunology
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Norwalk virus / pathogenicity
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Replicon
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Saliva / immunology
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Saliva / virology
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Vaccination
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Viral Vaccines / pharmacology
Substances
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ABO Blood-Group System
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Antibodies, Viral
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Viral Vaccines