Functional characterization of the human spermidine/spermine N(1)-acetyltransferase gene promoter

Hideyuki Tomitori; Mitsuru Nenoi; Kazuei Mita; Kazuhiro Daino; Kazuei Igarashi; Sachiko Ichimura

doi:10.1016/s0167-4781(02)00545-6

Functional characterization of the human spermidine/spermine N(1)-acetyltransferase gene promoter

Biochim Biophys Acta. 2002 Dec 12;1579(2-3):180-4. doi: 10.1016/s0167-4781(02)00545-6.

Authors

Hideyuki Tomitori¹, Mitsuru Nenoi, Kazuei Mita, Kazuhiro Daino, Kazuei Igarashi, Sachiko Ichimura

Affiliation

¹ Department of Clinical Biochemistry, Graduate School of Pharmaceutical Sciences, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba, Japan.

PMID: 12427553
DOI: 10.1016/s0167-4781(02)00545-6

Abstract

Spermidine/spermine N(1)-acetyltransferase (SSAT), the key enzyme of polyamine catabolism, is induced by antiproliferative stresses. We analyzed the 5' flanking region of the human SSAT gene, and clarified that the binding of Sp1 to the GC-box located 42 to 51 bp upstream from the transcription start site is essential for transcription in HeLa S3 cells. A polyamine-responsive element (PRE) seemed to be responsible for the elevated transcription after X-ray irradiation.

MeSH terms

5' Flanking Region
Acetyltransferases / biosynthesis
Acetyltransferases / genetics*
Acetyltransferases / metabolism
Blotting, Northern
DNA Footprinting
DNA-Binding Proteins
Dose-Response Relationship, Radiation
Electrophoretic Mobility Shift Assay
HeLa Cells / radiation effects
Humans
Point Mutation
Promoter Regions, Genetic / physiology*
Promoter Regions, Genetic / radiation effects
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Sp1 Transcription Factor / metabolism
Spermidine / metabolism*
Spermine / metabolism*
Time Factors
Transcription Initiation Site
Transcription, Genetic / radiation effects
Up-Regulation

Substances

DNA-Binding Proteins
RNA, Messenger
Sp1 Transcription Factor
Spermine
Acetyltransferases
diamine N-acetyltransferase
Spermidine