Abstract
High-frequent silencing of hematopoietic cell-specific protein-tyrosine phosphatase SHP1 gene by promoter methylation was detected in various kinds of leukemias and lymphomas, as well as in many hematopoietic cell lines, which is supported by our previous observation of strong decrease of SHP1 mRNA and protein. The promoter methylation of the SHP1 gene was clearly correlated with the clinical stage. Loss of heterozygosity with microsatellite markers near the SHP1 gene was shown in 79% of informative acute lymphoblastic leukemia cases. These results suggest that functional loss of SHP1 is associated with the pathogenesis of leukemias/lymphomas.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Base Sequence
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DNA Methylation*
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Gene Expression Regulation, Leukemic
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Gene Silencing*
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Humans
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Intracellular Signaling Peptides and Proteins
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Leukemia / enzymology
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Leukemia / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / enzymology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
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Leukemia, Myeloid / enzymology
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Leukemia, Myeloid / genetics
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Loss of Heterozygosity
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Lymphoma, T-Cell / enzymology
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Lymphoma, T-Cell / genetics*
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Molecular Sequence Data
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases / biosynthesis
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Protein Tyrosine Phosphatases / genetics*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Tumor Cells, Cultured
Substances
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Intracellular Signaling Peptides and Proteins
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RNA, Messenger
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PTPN6 protein, human
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases