Vaccination of mice against invasive aspergillosis with recombinant Aspergillus proteins and CpG oligodeoxynucleotides as adjuvants

Microbes Infect. 2002 Nov;4(13):1281-90. doi: 10.1016/s1286-4579(02)00007-2.

Abstract

In a murine model of invasive pulmonary aspergillosis, dendritic cells (DCs) pulsed with Aspergillus antigens induced the activation of CD4(+) Th1 cells capable of conferring resistance to the infection. Here we show that the combined, local delivery of unmethylated CpG oligodeoxynucleotides (ODNs) and the Asp f 16 Aspergillus allergen resulted in the functional maturation and activation of airway DCs capable of inducing Th1 priming and resistance to the fungus. Therefore, ODNs act as a potent adjuvant for the vaccine-induced protection against the fungus by promoting dominant Th1 response to Aspergillus antigens and allergens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Aspergillosis / immunology
  • Aspergillosis / pathology
  • Aspergillosis / prevention & control*
  • Aspergillus fumigatus / immunology*
  • Cells, Cultured
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Flow Cytometry
  • Fungal Proteins / genetics
  • Fungal Proteins / immunology
  • Fungal Vaccines*
  • Inflammation / immunology
  • Lung Diseases, Fungal / immunology
  • Lung Diseases, Fungal / pathology
  • Lung Diseases, Fungal / prevention & control*
  • Mice
  • Mice, Inbred BALB C
  • Oligodeoxyribonucleotides / pharmacology*
  • Recombinant Proteins / therapeutic use
  • Th1 Cells / immunology

Substances

  • Adjuvants, Immunologic
  • CPG-oligonucleotide
  • Fungal Proteins
  • Fungal Vaccines
  • Oligodeoxyribonucleotides
  • Recombinant Proteins