Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge

J Cell Physiol. 2003 Jan;194(1):45-53. doi: 10.1002/jcp.10185.

Abstract

Controlled regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped-bone with high-density bone trabeculae when used in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA-collagen sponge, show promise for use in a custom-shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants / trends
  • Animals
  • Antigens, Surface / metabolism
  • Biocompatible Materials / pharmacology
  • Biocompatible Materials / therapeutic use
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Bone Regeneration / drug effects
  • Bone Regeneration / physiology*
  • Cell Culture Techniques / methods*
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Collagen / pharmacology
  • Collagen / therapeutic use
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism
  • Female
  • Genes, Reporter
  • Lactic Acid / pharmacology
  • Lactic Acid / therapeutic use
  • Mice
  • Mice, Inbred C3H
  • Osteoblasts / cytology
  • Osteoblasts / metabolism*
  • Osteogenesis / drug effects
  • Osteogenesis / physiology
  • Polyglycolic Acid / pharmacology
  • Polyglycolic Acid / therapeutic use
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polymers / pharmacology
  • Polymers / therapeutic use
  • Stromal Cells / cytology
  • Stromal Cells / metabolism*
  • Tissue Engineering / methods*
  • Tissue Transplantation / methods*

Substances

  • Antigens, Surface
  • Biocompatible Materials
  • Polymers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Collagen