Identification of two genes differentially expressed upon different spatial configuration of the MGH-U3 human bladder cancer cells

Urol Oncol. 2002 Mar-Apr;7(2):57-61. doi: 10.1016/s1078-1439(01)00130-2.

Abstract

By using mRNA differential display technology, we have compared gene expression in neoconfluent (actively proliferating) and 14-day confluent (non-proliferative) MGH-U3 cells. Two clones (6.17.6 and 10.9.2) displayed significantly increased expression in the 14-day confluent MGH-U3 cells, as confirmed by Northern blot analysis. Nucleotide sequence showed that clone 6.17.6 was part of the human insulin-like growth factor binding protein-5 (IGFBP-5) cDNA, while clone 10.9.2 was part of a human gene with unknown function (clone HSI12602). The differential IGFBP-5 expression in this model system was also evidenced by Western analysis. The possible anti-proliferative role of IGFBP-5 in this model system is briefly discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Insulin-Like Growth Factor Binding Protein 5 / genetics
  • Molecular Sequence Data
  • RNA, Messenger / genetics*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / pathology*

Substances

  • Insulin-Like Growth Factor Binding Protein 5
  • RNA, Messenger
  • RNA, Neoplasm