Objective: To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.
Methods: Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).
Results: The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.
Conclusion: The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.