Abstract
To elucidate the induction of transmembrane signal transduction of keratinocytes by bullous pemphigoid (BP) antibodies, we assessed the intracellular Ca2+ concentration ([Ca2+]i) in DJM-1 cells (a squamous cell carcinoma cell line). The time course of fluo-3-fluorescence intensity was investigated, and transient increase of fluorescence intensity in DJM-1 cells was observed when the DJM-1 cells were incubated with rabbit IgG anti-human BP 180kDa antigen (BP 180). No increase of fluorescence intensity was observed when the cells were incubated with rabbit IgG anti-human BP 230 kDa antigen (BP 230). This transient increase of fluorescence intensity was inhibited by U-73122, a specific inhibitor of phospholipase C. These results indicate the possibility that an antibody to BP 180 induces transmembrane signal transduction in keratinocytes.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies / immunology*
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Autoantigens / administration & dosage
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Autoantigens / immunology*
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Calcium / metabolism*
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Carcinoma, Squamous Cell
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Carrier Proteins*
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Collagen / administration & dosage
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Collagen / immunology*
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Collagen Type XVII
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Cytoskeletal Proteins*
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Dystonin
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Fluorescent Antibody Technique, Indirect
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Humans
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Immunoglobulin G / blood
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Non-Fibrillar Collagens*
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Pemphigoid, Bullous / immunology*
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Rabbits
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Recombinant Fusion Proteins / administration & dosage
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Recombinant Fusion Proteins / immunology
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Signal Transduction*
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Tumor Cells, Cultured
Substances
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Antibodies
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Autoantigens
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Carrier Proteins
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Cytoskeletal Proteins
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DST protein, human
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Dystonin
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Immunoglobulin G
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Nerve Tissue Proteins
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Non-Fibrillar Collagens
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Recombinant Fusion Proteins
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Collagen
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Calcium