Abstract
Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity. Variation of the structure of the substrates, modification of the reaction system or protein engineering (e.g. the expression of pure enzymes, rational design or directed evolution) are strategies that can be employed to improve the distinction between two enantiomers or enantiotopic groups.
MeSH terms
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Enzyme Activation
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Esterases / biosynthesis*
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Esterases / chemistry*
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Esterases / classification
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Esterases / genetics
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Evolution, Molecular
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Gene Expression Regulation, Fungal*
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Isoenzymes / biosynthesis
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Isoenzymes / chemistry
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Isoenzymes / classification
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Isoenzymes / genetics
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Lipase / biosynthesis*
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Lipase / chemistry*
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Lipase / classification
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Lipase / genetics
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Molecular Conformation
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Protein Engineering / methods*
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Quality Control
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Solvents / chemistry
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Substrate Specificity
Substances
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Isoenzymes
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Solvents
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Esterases
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Lipase