Abstract
We isolated two mouse cDNA clones which show significant similarities with human angiotensin-converting enzyme-related carboxypeptidase (ACE2). The cDNAs were 2746 and 1995 bp in length and seemed to arise from the same gene by alternative splicing. The longer cDNA encoded a 798-amino acid protein containing the sequence motif conserved among zinc metallopeptidases. Mouse ACE2 showed 83% identity with human ACE2. Northern blot analysis revealed that 2.8- and 2.0-kb transcripts were expressed mainly in the kidney and the lungs. The mouse ACE2 gene was mapped to chromosome X 70.5 cM.
MeSH terms
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Amino Acid Sequence
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Angiotensin-Converting Enzyme 2
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Animals
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Base Sequence
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Blotting, Northern
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Carboxypeptidases / biosynthesis
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Carboxypeptidases / genetics*
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Chromosome Mapping
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Chromosomes, Human, X*
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Cloning, Molecular
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Gene Expression Regulation, Enzymologic
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Humans
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Kidney
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Lung
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Mice
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Molecular Sequence Data
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Open Reading Frames
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Peptidyl-Dipeptidase A
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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Carboxypeptidases
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Peptidyl-Dipeptidase A
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ACE2 protein, human
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Ace2 protein, mouse
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Angiotensin-Converting Enzyme 2
Associated data
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GENBANK/AB053181
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GENBANK/AB053182