Dose-effect relations of loop- and thiazide-diuretics on calcium homeostasis: a randomized, double-blinded Latin-square multiple cross-over study in postmenopausal osteopenic women

Eur J Clin Invest. 2003 Jan;33(1):41-50. doi: 10.1046/j.1365-2362.2003.01103.x.

Abstract

Background: Thiazide diuretics (TDs) reduce whereas loop diuretics (LDs) increase urinary calcium. We studied the effects of different doses of a TD and LD on electrolytes, calcitropic hormones and biochemical bone markers.

Subjects and methods: In a five-period crossover study, comparing four active doses with placebo, 40 postmenopausal women with osteopenia were treated with different doses of LD bumetanide (n = 20, 0.5-2.0 mg per day) or TD bendroflumethiazide (n = 20, 2.5-10 mg per day). Each treatment period lasted 1 week.

Results: Urinary calcium decreased dose-dependently in response to the bendroflumethiazide. The best hypocalciuric effect was achieved by 5 mg day-1 of bendroflumethiazide. Total plasma calcium levels increased, whereas ionised calcium at ambient pH-values decreased because of increased pH-values in response to the bendroflumethiazide. Plasma PTH levels did not change, whereas a slight dose-dependent increase occurred in plasma 1,25(OH)2D levels. As a marker of bone formation, plasma osteocalcin levels increased. Conversely, bumetanide dose-dependently increased renal calcium losses with a concomitant increase in plasma PTH and 1,25(OH)2D levels. Plasma osteocalcin levels increased and bone-specific alkaline phosphatase levels decreased dose-dependently.

Conclusion: Whether a LD or TD is chosen as diuretic therapy affects calcium homeostasis. The effects of LDs are potentially harmful to bone. Further studies are needed to evaluate whether long-term treatment with LDs causes osteoporosis. Until then, we suggest using, if possible, a TD rather than a LD as diuretic therapy in order not to risk deleterious effects on bone metabolism.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial

MeSH terms

  • Alkaline Phosphatase / blood
  • Bendroflumethiazide / pharmacology
  • Biomarkers / blood
  • Bone Diseases, Metabolic / metabolism
  • Bone Diseases, Metabolic / physiopathology*
  • Bumetanide / pharmacology
  • Calcium / metabolism*
  • Cross-Over Studies
  • Diuretics / pharmacology*
  • Dose-Response Relationship, Drug
  • Double-Blind Method
  • Female
  • Homeostasis / drug effects*
  • Humans
  • Middle Aged
  • Osteocalcin / blood
  • Parathyroid Hormone / blood
  • Sodium Chloride Symporter Inhibitors / pharmacology*

Substances

  • Biomarkers
  • Diuretics
  • Parathyroid Hormone
  • Sodium Chloride Symporter Inhibitors
  • Bumetanide
  • Osteocalcin
  • Bendroflumethiazide
  • Alkaline Phosphatase
  • Calcium