Abstract
We present a 3D double sensitivity enhanced X-filtered homonuclear TOCSY-TOCSY experiment for the assignment of unlabeled molecules complexed to labeled protein- or nucleic acid-domains. The resulting spectrum is clean, can be measured in a reasonable amount of time and allows for increased resolution of overlapping resonances when compared to 2D methods. The 3D X-filtered TOCSY-TOCSY allows for assignment in cases where the size or the composition of the unlabeled molecule results in a high degree of overlap.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Cycle Proteins
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Dimerization
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Image Processing, Computer-Assisted / methods
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Imaging, Three-Dimensional* / methods
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Molecular Sequence Data
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Nuclear Proteins
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Peptide Fragments / chemistry
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Phosphoproteins / chemistry
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Protein Conformation
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Protein Structure, Tertiary
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Repressor Proteins / chemistry
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Sensitivity and Specificity
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Transcription Factors / chemistry
Substances
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Cell Cycle Proteins
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Nuclear Proteins
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Peptide Fragments
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Phosphoproteins
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Repressor Proteins
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Transcription Factors