Detection of avian pneumovirus in wild Canada (Branta canadensis) and blue-winged teal (Anas discors) geese

Avian Dis. 2002 Oct-Dec;46(4):1025-9. doi: 10.1637/0005-2086(2002)046[1025:DOAPIW]2.0.CO;2.

Abstract

Choanal cleft swab samples from 770 wild Canada geese (Branta canadensis) and 358 blue-winged teal (Anas discors), captured for relocation or banding, were examined for the presence of avian pneumovirus (APV) RNA by reverse transcription (RT)-polymerase chain reaction (PCR) and for virus isolation. The swab samples were pooled into groups of 5 or 10. Sixty eight of 102 (66.7%) pooled goose samples were RT-PCR positive for APV RNA. Thirteen of 52 (25.0%) pooled blue-winged teal samples were RT-PCR positive for APV RNA. APV RNA-positive samples were inoculated onto chick embryo fibroblasts (CEF) and QT-35 cells. Infectious APV was isolated from five Canada goose pooled samples in CEF and from one Canada goose pool in QT-35 cells but not from blue-winged teal.

MeSH terms

  • Animals
  • Animals, Wild / virology
  • Ducks / virology*
  • Fluorescent Antibody Technique, Indirect
  • Geese / virology*
  • Pneumovirus / classification
  • Pneumovirus / isolation & purification*
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Viral