Concentration-dependent dual effect of anandamide on sensory neuropeptide release from isolated rat tracheae

Neurosci Lett. 2003 Jan 16;336(2):89-92. doi: 10.1016/s0304-3940(02)01221-1.

Abstract

Most actions of anandamide (AEA) are mediated by the cannabinoid 1 (CB(1)) receptor activation, but on sensory neurones it is also an agonist on the vanilloid subtype 1 receptor (VR(1)). The aim of the present study was to analyse the effect of AEA (10(-6)-10(-4) M) on inhibitory CB(1) and excitatory VR(1) receptors by measuring sensory neuropeptide release such as somatostatin, substance P and calcitonin gene-related peptide, from isolated rat tracheae. AEA (10(-6) M) vas without significant effect, 10(-5) M inhibited neuropeptide release, which was abolished by the G protein-coupled receptor blocker pertussis toxin (100 ng/ml) and the CB(1) receptor antagonist SR141716A (5x10(-7) M). High concentrations of AEA (5x10(-5) M, 10(-4) M) increased the release of the peptides and this inhibition was prevented by the competitive VR(1) antagonist capsazepine (10(-5) M). These results indicate a dual, concentration-dependent action of AEA on CB(1) receptors and VR(1) on peripheral sensory nerve terminals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acids / administration & dosage*
  • Arachidonic Acids / metabolism
  • Calcitonin Gene-Related Peptide / metabolism
  • Capsaicin / analogs & derivatives*
  • Capsaicin / metabolism
  • Capsaicin / pharmacology
  • Dose-Response Relationship, Drug
  • Endocannabinoids
  • In Vitro Techniques
  • Male
  • Neuropeptides / drug effects
  • Neuropeptides / metabolism*
  • Pertussis Toxin / metabolism
  • Pertussis Toxin / pharmacology
  • Piperidines / metabolism
  • Piperidines / pharmacology
  • Polyunsaturated Alkamides
  • Pyrazoles / metabolism
  • Pyrazoles / pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Cannabinoid
  • Receptors, Drug / agonists*
  • Reference Values
  • Reproducibility of Results
  • Rimonabant
  • Sensitivity and Specificity
  • Somatostatin / metabolism
  • Substance P / metabolism
  • Trachea / drug effects
  • Trachea / metabolism*

Substances

  • Arachidonic Acids
  • Endocannabinoids
  • Neuropeptides
  • Piperidines
  • Polyunsaturated Alkamides
  • Pyrazoles
  • Receptors, Cannabinoid
  • Receptors, Drug
  • Substance P
  • Somatostatin
  • Pertussis Toxin
  • Calcitonin Gene-Related Peptide
  • capsazepine
  • Rimonabant
  • Capsaicin
  • anandamide