Abstract
Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Caveolin 1
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Caveolins / analysis
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Cells, Cultured
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Fixatives
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Formaldehyde
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Frozen Sections*
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Humans
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Microscopy, Electron
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Microscopy, Fluorescence
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Microscopy, Immunoelectron / methods*
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Placenta / chemistry
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Placenta / ultrastructure
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Polymers
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Staining and Labeling / methods*
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Umbilical Veins / chemistry
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Umbilical Veins / cytology
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Umbilical Veins / ultrastructure
Substances
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Caveolin 1
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Caveolins
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Fixatives
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Polymers
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Formaldehyde
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paraform