Abstract
Interleukin-25 (IL-25) is a recently described T helper 2 (T(H)2) cell-derived cytokine that belongs to the IL-17 family and induces the production of IL-4, IL-5, and IL-13 from an unidentified non-T-cell population. Here, we show that mast cells are also potent IL-25-producing cells. When bone marrow-derived mast cells were stimulated by immunoglobulin E cross-linking, IL-25 mRNA was induced within 30 minutes in a calcineurin-dependent manner, and the levels of IL-25 mRNA were comparable with those of activated T(H)2 cells. Production of IL-25 by mast cells was also detected at protein levels by immunoblotting. These results suggest that mast cells may enhance T(H)2-type immune response by producing IL-25.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcimycin / pharmacology
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Calcineurin / physiology
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Gene Expression Regulation / drug effects*
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Immunoglobulin E / pharmacology*
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Interleukin-17 / biosynthesis
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Interleukin-17 / genetics
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Interleukins / biosynthesis*
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Interleukins / genetics
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Mast Cells / drug effects
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Mast Cells / metabolism*
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Mice
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RNA, Messenger / biosynthesis
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Receptors, IgE / physiology*
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Reverse Transcriptase Polymerase Chain Reaction
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Tetradecanoylphorbol Acetate / pharmacology
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Th2 Cells / metabolism
Substances
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IL17F protein, human
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IL25 protein, human
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Interleukin-17
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Interleukins
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Mydgf protein, mouse
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RNA, Messenger
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Receptors, IgE
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Immunoglobulin E
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Calcimycin
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Calcineurin
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Tetradecanoylphorbol Acetate