Abstract
We identified an open reading frame from a database of the entire genome of Synechococcus elongatus, the product of which was very similar to pixJ1, which was proposed as photoreceptor gene for phototaxis in Synechocystis sp. PCC6803 [Yoshihara et al. (2000) Plant Cell Physiol. 41: 1299]. The mRNA of S. elongatus pixJ (SepixJ) was expressed in vivo as a part of the product of an operon. SePixJ was detected exclusively in the membrane fraction after cell fractionation. Immunogold labeling of SePixJ in ultra-thin sections indicated that it existed only in both ends of the rod-shaped cell; probably bound with the cytoplasmic membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cyanobacteria / genetics*
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Cyanobacteria / physiology
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Cyanobacteria / radiation effects
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Gene Expression Regulation, Bacterial / radiation effects
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Light
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Microscopy, Immunoelectron
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Molecular Sequence Data
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Photoreceptors, Microbial / genetics*
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Photoreceptors, Microbial / metabolism
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Photosynthetic Reaction Center Complex Proteins / metabolism
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Photosynthetic Reaction Center Complex Proteins / radiation effects
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Phytochrome / metabolism
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Sequence Homology, Amino Acid
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Signal Transduction / radiation effects
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Temperature
Substances
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Bacterial Proteins
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Photoreceptors, Microbial
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Photosynthetic Reaction Center Complex Proteins
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Phytochrome