Abstract
In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase(MTHFR) gene in Epstein-Barr virus(EBV) transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT-PCR protocol and analysed by polyacrylamide gel electrophoresis(PAGE). One of the cDNA segments was sequenced. Results showed MTHFR gene was expressed in human B lymphoblast cell line. The cDNA sequence was almost the same as that of human liver cDNA from Genbank.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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B-Lymphocytes / cytology
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B-Lymphocytes / metabolism*
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Base Sequence
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Cell Line
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Cell Transformation, Viral*
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DNA, Complementary / genetics
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Herpesvirus 4, Human / genetics*
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Humans
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Methylenetetrahydrofolate Reductase (NADPH2)
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Molecular Sequence Data
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Oxidoreductases Acting on CH-NH Group Donors / biosynthesis*
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Oxidoreductases Acting on CH-NH Group Donors / genetics
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Sequence Analysis, DNA
Substances
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DNA, Complementary
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RNA, Messenger
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Oxidoreductases Acting on CH-NH Group Donors
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Methylenetetrahydrofolate Reductase (NADPH2)