Allele and genotype frequencies of four short tandem repeat loci were determined in a population sample from east China by using PCR and fluorescence techniques. After denaturing PAGE, 9 alleles were identified for CSF1PO, 8 alleles for TPOX, and 7 alleles for TH01. All loci met Hardy-Weinberg expectations. In addition, the theoretical values of these loci for paternity and forensic casework were evaluated.