Objective: To determine the role of middle ear epithelial cell proliferation and apoptosis in the pathogenesis of human cholesteatoma.
Method: 33 cholesteatoma, 25 auditory meatal skin of cholesteatoma and 10 normal human auditory meatal skin were detected by using immunohistochemistry analysis for cell proliferation with Ki 67, PCNA and PCNA antibody and TUNTEL methods for cell apoptosis.
Result: PCNA expression and apoptosis cells exists different quantity in cholesteatoma, auditory metal skin of cholesteatoma and normal human auditory meatal skin and distributes in various cell layer. There were positive correlation between p53 and PCNA as well as negative correlation between p53 and apoptosis.
Conclusion: The dyregulation of proliferation and apoptosis in keratinocyte is important in pathogenesis of cholesteatoma.