New HLA-DRB1*07 allele identified from microlymphocytotoxicity/SSP discrepancies

Tissue Antigens. 2002 Dec;60(6):539-41. doi: 10.1034/j.1399-0039.2002.600611.x.

Abstract

A HLA-DRB1*07 variant allele has been identified in a cadaver kidney donor. Serological typing using monoclonal antibodies detected HLA-DR4 and HLA-DR7. HLA class II DNA typing using sequence-specific primer (PCR-SSP) polymerase chain reaction only detected DRB1*04, while sequence-specific oligonucleotide (PCR-SSO) polymerase chain reaction confirmed the presence of both DRB1*04 and DRB1*07 alleles, although two extra reactions were also found. Exon 2 of the HLA-DRB1*07 was isolated using allele-specific PCR, then cloned and sequenced. Four mutations, at positions 170 (T --> C), 171 (C --> T), 174 (C --> G), and 179 (C --> A), were observed. These mutations changed codons 57 and 60 (V --> A; S --> Y, respectively). This amino acid sequence at position 56-61 is only found in DRB1*0811.

MeSH terms

  • Alleles
  • Base Sequence
  • Cytotoxicity Tests, Immunologic
  • Exons
  • HLA-DR Antigens / chemistry
  • HLA-DR Antigens / genetics*
  • HLA-DR Antigens / immunology
  • HLA-DRB1 Chains
  • Histocompatibility Testing
  • Humans
  • Molecular Sequence Data
  • Protein Structure, Secondary

Substances

  • HLA-DR Antigens
  • HLA-DRB1 Chains