Abstract
Since denditric cells (DC) represent the main players linking innate and adaptive immunity, their prompt generation from blood cells would be instrumental for an efficient immune response to infections. Consistent with this, CD2+ monocytes were found to express the DC maturation marker CD83, along with acquisition of high antigen-presenting activity, after a surprisingly short time in culture. This rapid process is associated with expression of IFN-alpha/beta genes and secretion of low levels of pro-inflammatory cytokines. Exposure of monocytes to IFN-alpha, but not to IL-4, induced persistence of CD2+/CD83+ cells, which were fully competent in stimulating primary responses by naive T cells. These results unravel the natural pathway by which infection-induced signals rapidly transform pre-armed monocytes into active DC.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antigen Presentation
-
Antigens, CD
-
CD2 Antigens / biosynthesis*
-
CD2 Antigens / genetics
-
CD4-Positive T-Lymphocytes / immunology
-
CD83 Antigen
-
Cell Differentiation
-
Cytokines / metabolism
-
Dendritic Cells / classification*
-
Dendritic Cells / drug effects
-
Dendritic Cells / immunology
-
Dendritic Cells / metabolism
-
Gene Expression Regulation / drug effects
-
HIV Antigens / immunology
-
HIV-1 / immunology
-
Humans
-
Immunoglobulins / biosynthesis*
-
Immunoglobulins / genetics
-
Interferon Type I / biosynthesis
-
Interferon Type I / metabolism
-
Interferon-alpha / pharmacology
-
Interleukin-4 / pharmacology
-
Lipopolysaccharide Receptors / biosynthesis*
-
Lipopolysaccharide Receptors / genetics
-
Lymphocyte Culture Test, Mixed
-
Membrane Glycoproteins / biosynthesis*
-
Membrane Glycoproteins / genetics
-
Monocytes / cytology
-
Monocytes / drug effects*
Substances
-
Antigens, CD
-
CD2 Antigens
-
Cytokines
-
HIV Antigens
-
Immunoglobulins
-
Interferon Type I
-
Interferon-alpha
-
Lipopolysaccharide Receptors
-
Membrane Glycoproteins
-
Interleukin-4