Electron microscopy of functional ribosome complexes

Biopolymers. 2003 Feb;68(2):223-33. doi: 10.1002/bip.10210.

Abstract

Cryoelectron microscopy has made a number of significant contributions to our understanding of the translation process. The method of single-particle reconstruction is particularly well suited for the study of the dynamics of ribosome-ligand interactions. This review follows the events of the functional cycle and discusses the findings in the context provided by the recently published x-ray structures.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Cryoelectron Microscopy
  • Image Processing, Computer-Assisted
  • RNA, Ribosomal, 16S / ultrastructure
  • RNA, Transfer / ultrastructure
  • RNA, Transfer, Met / ultrastructure*
  • Ribosomes / ultrastructure*

Substances

  • RNA, Ribosomal, 16S
  • RNA, Transfer, Met
  • fMet-tRNA(fMet)
  • RNA, Transfer