Abstract
The effect of PIC-BE on the expression of mdr-1, bcl-2 and bax genes and their protein products (P-gp, Bcl-2 and Bax) was observed respectively in a multidrug resistance (MDR) cell variant K562/ADM. The results showed that PIC-BE could significantly inhibit the expression of mdr-1 and bcl-2 genes at both mRNA and protein levels in K562/ADM cell line, and the effect was dose- and time-dependent within limited range. Under same condition, although PIC-BE could increase the expression of Bax slightly, there was no statistically significant difference. These results suggest that the reversal of the MDR of K562/ADM cell line by PIC-BE may result from its effect on the expression of mdr-1, bcl-2 genes and their protein products.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
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Antineoplastic Agents / pharmacology*
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Dose-Response Relationship, Drug
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Doxorubicin / pharmacology
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Drug Resistance, Multiple / genetics*
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Drug Resistance, Neoplasm / genetics*
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Gene Expression / drug effects
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Genes, MDR*
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Genes, bcl-2*
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Humans
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K562 Cells / drug effects
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Plant Extracts / pharmacology*
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis
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Sesquiterpenes*
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bcl-2-Associated X Protein
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents
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BAX protein, human
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Plant Extracts
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Sesquiterpenes
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bcl-2-Associated X Protein
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beta-elemene
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Doxorubicin