[Determination of dyes in cosmetic by high performance liquid chromatography]

Se Pu. 1999 Sep;17(5):486-7.
[Article in Chinese]

Abstract

A reversed-phase high performance liquid chromatographic method was used to detect 5 dyes including p-phenylenediamine in cosmetrics. A Zorbax C8 column was used and mobile phase was V (triethanolamine):V(water):V(acetonitrile) = 0.95:94.05:5 adjusted to pH 7.7 by phosphoric acid. The wavelength 280 nm was selected. Samples were extracted with 95% ethanol by ultrasonic method. The recoveries were 87%-107% and the relative standard deviations were 2.3%-6.4%.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Coloring Agents / analysis*
  • Hair Dyes / analysis*
  • Hydroquinones / analysis
  • Phenylenediamines / analysis*

Substances

  • Coloring Agents
  • Hair Dyes
  • Hydroquinones
  • Phenylenediamines
  • 4-phenylenediamine
  • hydroquinone