Over expression of bone morphogenetic protein-3b (BMP-3b) using an adenoviral vector promote the osteoblastic differentiation in C2C12 cells and augment the bone formation induced by bone morphogenetic protein-2 (BMP-2) in rats

Shinji Kaihara; Kazuhisa Bessho; Yasunori Okubo; Junya Sonobe; Yasato Komatsu; Masako Miura; Shin-Ichi Miyatake; Kazuwa Nakao; Tadahiko Iizuka

doi:10.1016/s0024-3205(02)02477-3

Over expression of bone morphogenetic protein-3b (BMP-3b) using an adenoviral vector promote the osteoblastic differentiation in C2C12 cells and augment the bone formation induced by bone morphogenetic protein-2 (BMP-2) in rats

Life Sci. 2003 Feb 28;72(15):1683-93. doi: 10.1016/s0024-3205(02)02477-3.

Authors

Shinji Kaihara¹, Kazuhisa Bessho, Yasunori Okubo, Junya Sonobe, Yasato Komatsu, Masako Miura, Shin-Ichi Miyatake, Kazuwa Nakao, Tadahiko Iizuka

Affiliation

¹ Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo, Kyoto 606-8507, Japan.

PMID: 12559390
DOI: 10.1016/s0024-3205(02)02477-3

Abstract

BMP-3b is a novel BMP-3-related protein and its biological functions are unknown. In order to investigate the biological actions of BMP-3b, we constructed a BMP-3b-expressing recombinant adenoviral vector (AxCAKBMP-3b). We show that over expression of BMP-3b stimulated the induction of differentiation and the osteoinduction activity of a human BMP-2-expressing recombinant adenoviral vector (AxCAOBMP-2). C2C12 cells were infected in vitro with AxCAKBMP-3b, AxCAOBMP-2 and a control vector containing no foreign genes (AxCAwt). Cells infected with AxCAOBMP-2 and AxCAKBMP-3b produced more alkaline phosphatase and secreted more osteocalcin into the culture medium than cells infected with AxCAOBMP-2 and AxCAwt. When AxCAOBMP-2, AxCAKBMP-3b, and AxCAwt were injected into the calf muscles of nude rats (F 344/N Jcl-rnu), the osteoinduction seen with AxCAOBMP-2 and AxCAKBMP-3b was greater than that seen with AxCAOBMP-2 and AxCAwt.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae / genetics*
Alkaline Phosphatase / metabolism
Animals
Blotting, Western
Bone Matrix / anatomy & histology
Bone Matrix / growth & development
Bone Matrix / physiology
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 3
Bone Morphogenetic Proteins / biosynthesis*
Bone Morphogenetic Proteins / genetics
Bone Morphogenetic Proteins / pharmacology*
Calcium / metabolism
Cell Differentiation / drug effects
Cell Line
Gene Expression Regulation / drug effects*
Genetic Vectors / genetics*
Growth Differentiation Factor 10
Humans
Male
Osteoblasts / drug effects*
Osteocalcin / biosynthesis
Rats
Rats, Inbred F344
Recombinant Proteins
Transforming Growth Factor beta*

Substances

BMP2 protein, human
BMP3 protein, human
Bmp2 protein, rat
Bmp3 protein, rat
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 3
Bone Morphogenetic Proteins
GDF10 protein, human
Gdf10 protein, rat
Growth Differentiation Factor 10
Recombinant Proteins
Transforming Growth Factor beta
recombinant human bone morphogenetic protein-2
Osteocalcin
Alkaline Phosphatase
Calcium