Aim: To investigate the dynamics of the anti-GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum.
Methods: The specific antibodies were detected weekly by GST-ELISA in rabbits vaccinated with purified recombinant 26 kDa GST antigen of S. japonicum plus FCA/IFCA; rabbits immunized with 0.85% saline plus FCA/IFCA served as control group.
Results: The specific anti-GST antibodies began to increase at week 4 post-immunization, the mean OD value in rabbits vaccinated with recombinant GST could reach 1.03 +/- 0.46 (adjuvant control group only 0.42 +/- 0.04). The experiment was completed at week 65 post-immunization when the mean OD values for specific anti-GST antibody in immunized rabbits were 0.94 +/- 0.26(0.29 +/- 0.16 in the control group).
Conclusion: Strong anti-GST antibody responses could be induced in rabbits vaccinated with recombinant 26 kDa GST of S. japonicum.