Chemoenzymatic synthesis of high-mannose type HIV-1 gp120 glycopeptides

Suddham Singh; Jiahong Ni; Lai-Xi Wang

doi:10.1016/s0960-894x(02)01025-9

Chemoenzymatic synthesis of high-mannose type HIV-1 gp120 glycopeptides

Bioorg Med Chem Lett. 2003 Feb 10;13(3):327-30. doi: 10.1016/s0960-894x(02)01025-9.

Authors

Suddham Singh¹, Jiahong Ni, Lai-Xi Wang

Affiliation

¹ Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 West Lombard Street, Baltimore, MD 21201, USA.

PMID: 12565922
DOI: 10.1016/s0960-894x(02)01025-9

Abstract

A chemoenzymatic approach to the synthesis of glycoforms of HIV-1 gp120 glycopeptides is described. Thus, the high-mannose type glycopeptides [gp120 (336-342)] containing Man(9), Man(6) and Man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant Arthrobacter Endo-beta-N-acetyl-glucosaminidase (Endo-A) as the key enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Chromatography, High Pressure Liquid
Glycopeptides / chemical synthesis*
Glycopeptides / chemistry
HIV Envelope Protein gp120 / chemistry*
HIV-1 / chemistry
Mannose / chemistry*
Molecular Sequence Data
Oligosaccharides / chemical synthesis

Substances

Glycopeptides
HIV Envelope Protein gp120
Oligosaccharides
Mannose