[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies]

L S Qiu; Z Feng; Y H Zhang; H Li

[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies]

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(2):92-3.

[Article in Chinese]

Authors

L S Qiu¹, Z Feng, Y H Zhang, H Li

Affiliation

¹ Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.

PMID: 12567723

Abstract

Objective: To detect circulating antigen in the patients with schistosomiasis.

Methods: Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA.

Results: Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.

Conclusion: PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal
Antigens, Helminth / blood*
Enzyme-Linked Immunosorbent Assay / methods
Humans
Schistosoma japonicum / immunology*
Schistosomiasis japonica / diagnosis
Schistosomiasis japonica / immunology*
Sensitivity and Specificity

Substances

Antibodies, Monoclonal
Antigens, Helminth