Mutation analysis of BrCA1, BrCA2, and p53 versus soluble HLA class I and class II in a case of familial endometriosis

Fertil Steril. 2003 Feb;79(2):445-8. doi: 10.1016/s0015-0282(02)04665-4.

Abstract

Objective: To investigate possible correlation(s) between mutations of BrCA1, BrCA2, and p53 genes versus soluble HLA expression in familial endometriosis.

Design: Mutation analysis.

Setting: University teaching departments and hospital.

Patient(s): A family with seven women in two generations with familial endometriosis.

Intervention(s): Mutation analysis of BrCA1, BrCA2, and p53 genes.

Main outcome measure(s): A point mutation of the BrCA1 gene appears to inhibit soluble HLA secretion.

Result(s): Among the three genes examined, only the BrCA1 gene showed a T to A mutation at position 3232 that correlates with total abolishment of both class I and class II antigen release.

Conclusion(s): A possible correlation between a BrCA1 mutation and soluble HLA expression appears to exist. The mutation is not stage dependent and seemingly influences the secretion of both class I and class II antigens that are totally absent from the serum of only one family member.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Base Sequence
  • DNA Mutational Analysis
  • DNA Primers
  • Endometriosis / genetics*
  • Exons
  • Female
  • Genes, BRCA1*
  • Genes, BRCA2*
  • Genes, p53*
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class II / genetics*
  • Humans
  • Major Histocompatibility Complex
  • Middle Aged
  • Point Mutation*
  • Polymerase Chain Reaction

Substances

  • DNA Primers
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II