The volatile oil of Baizhu was included with beta-CD. The factors affecting the formation of the inclusion compounds were researched by L9(3(4)) orthogonal test. The ratio of the oil in inclusion compounds and recovery of inclusion compounds were used as criteria in the test. The best conditions for inclusion were A2B3C1, that is, the compounds should be formed in the aqueous solution containing beta-CD and oil with a raio of 8:1, aqua destillate and beta-CD with a ratio of 4:1 for two hours.