Abstract
Burkholderia pseudomallei and Burkholderia thailandensis express similar O-antigens (O-PS II) in which their 6-deoxy-alpha-L-talopyranosyl (L-6dTalp) residues are variably substituted with O-acetyl groups at the O-2 or O-4 positions. In previous studies we demonstrated that the protective monoclonal antibody, Pp-PS-W, reacted with O-PS II expressed by wild-type B. pseudomallei strains but not by a B. pseudomallei wbiA null mutant. In the present study we demonstrate that WbiA activity is required for the acetylation of the L-6dTalp residues at the O-2 position and that structural modification of O-PS II molecules at this site is critical for recognition by Pp-PS-W.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acetylation
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Amino Acid Sequence
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Burkholderia / genetics
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Burkholderia / immunology
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Burkholderia / metabolism*
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Burkholderia pseudomallei / genetics
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Burkholderia pseudomallei / immunology
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Burkholderia pseudomallei / metabolism*
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DNA, Bacterial / genetics
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Epitopes / immunology
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Genes, Bacterial*
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Humans
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Lipopolysaccharides / metabolism
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Locus Control Region
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Magnetic Resonance Spectroscopy
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Models, Molecular
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Molecular Sequence Data
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O Antigens / chemistry
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O Antigens / immunology
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O Antigens / metabolism*
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Sequence Alignment
Substances
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DNA, Bacterial
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Epitopes
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Lipopolysaccharides
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O Antigens