The novel differentiation of human blood mononuclear cells into CD1a-negative dendritic cells is stimulated in the absence of exogenous cytokines by an extract prepared from pinecones

Int Immunopharmacol. 2003 Feb;3(2):209-23. doi: 10.1016/S1567-5769(02)00267-9.

Abstract

The production of dendritic cells, both in-vivo and in-vitro, has become the intense focus of research activities. Common to many of these production protocols is the use of cytokines, typically granulocyte-monocyte colony stimulating factor and either interleukin 4 or tumor necrosis factor alpha or a combination of all three. Herein, we report our findings that a proprietary pinecone extract is capable of in a dose-dependent manner, and in the absence of exogenous cytokines, the rapid differentiation from peripheral blood mononuclear cells of mature CD1a-negative dendritic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD1 / analysis*
  • Cell Adhesion / drug effects
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / cytology*
  • Endotoxins / pharmacology
  • Female
  • Gene Expression / drug effects
  • Humans
  • Immunohistochemistry
  • Leukocytes, Mononuclear / cytology*
  • Lipopolysaccharide Receptors / analysis
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Oligodeoxyribonucleotides / pharmacology
  • Pinus / chemistry*
  • Plant Extracts / pharmacology*

Substances

  • Antigens, CD1
  • CD1a antigen
  • CPG-oligonucleotide
  • Endotoxins
  • Lipopolysaccharide Receptors
  • Oligodeoxyribonucleotides
  • Plant Extracts