Abstract
Small polybasic peptides derived from the transduction domains of certain proteins, such as the third alpha-helix of the Antennapedia (Antp) homeodomain, can cross the cell membrane through a receptor-independent mechanism. These cell-permeable molecules have been used as 'Trojan horses' to introduce biologically active cargo molecules such as DNA, peptides or proteins into cells. Using these cell-permeable peptides, we have developed an efficient and simple method to increase virally mediated gene delivery and protein expression in vitro and in vivo. Here, we show that cell-permeable peptides increase viral cell entry, improve gene expression at reduced titers of virus and improve efficacy of therapeutically relevant genes in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / metabolism
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Amino Acid Sequence
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Animals
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Antennapedia Homeodomain Protein
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Arteries / cytology
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Arteries / metabolism
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COS Cells
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Fluorescent Dyes / metabolism
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Gene Expression Regulation
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Gene Transfer Techniques*
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Genetic Therapy
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Hindlimb / blood supply
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Humans
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Ischemia
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Molecular Sequence Data
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Muscle, Skeletal / metabolism
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Nitric Oxide Synthase / genetics
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type III
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Nuclear Proteins*
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Peptides / chemistry
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Peptides / genetics
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Peptides / metabolism*
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Transcription Factors*
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Virus Replication* / physiology*
Substances
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Antennapedia Homeodomain Protein
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Fluorescent Dyes
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Homeodomain Proteins
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Nuclear Proteins
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Peptides
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Recombinant Fusion Proteins
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Transcription Factors
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NOS3 protein, human
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type III