WT-1 and NPHS2 mutation analysis in patients with non-familial steroid-resistant focal-segmental glomerulosclerosis

Clin Nephrol. 2003 Feb;59(2):143-6. doi: 10.5414/cnp59143.

Abstract

Background: Familial forms of steroid-resistant nephrotic syndrome with the histologic findings of focal-segmental glomerulosclerosis have frequently a genetic basis. For the non-familial forms this is still unresolved.

Patients and methods: Ten children with non-familial steroid-resistant nephrotic syndrome along with focal-segmental glomerulosclerosis were tested for mutations in the WT-1 and NPHS2 genes.

Results: In 1 patient, a mutation in intron 9 of the WT-1 gene and in 1 patient a heterozygous NPHS2 mutation could be detected. Both abnormalities are important for the treatment modalities and prognosis.

Conclusion: Additional studies will have to provide a solid basis for the recommendation of mutation analysis in non-familial steroid-resistant focal-segmental glomerulosclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • DNA Mutational Analysis
  • Female
  • Glomerulosclerosis, Focal Segmental / genetics
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Introns
  • Male
  • Membrane Proteins / genetics*
  • Nephrotic Syndrome / genetics*
  • Polymorphism, Genetic
  • WT1 Proteins / genetics*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • NPHS2 protein
  • WT1 Proteins