A plant-specific dynamin-related protein forms a ring at the chloroplast division site

Plant Cell. 2003 Mar;15(3):655-65. doi: 10.1105/tpc.009373.

Abstract

Chloroplasts have retained the bacterial FtsZ for division, whereas mitochondria lack FtsZ except in some lower eukaryotes. Instead, mitochondrial division involves a dynamin-related protein, suggesting that chloroplasts retained the bacterial division system, whereas a dynamin-based system replaced the bacterial system in mitochondria during evolution. In this study, we identified a novel plant-specific group of dynamins from the primitive red alga Cyanidioschyzon merolae. Synchronization of chloroplast division and immunoblot analyses showed that the protein (CmDnm2) associates with the chloroplast only during division. Immunocytochemical analyses showed that CmDnm2 appears in cytoplasmic patches just before chloroplast division and is recruited to the cytosolic side of the chloroplast division site to form a ring in the late stage of division. The ring constricts until division is complete, after which it disappears. These results show that a dynamin-related protein also participates in chloroplast division and that its behavior differs from that of FtsZ and plastid-dividing rings that form before constriction at the site of division. Combined with the results of a recent study of mitochondrial division in Cyanidioschyzon, our findings led us to hypothesize that when first established in lower eukaryotes, mitochondria and chloroplasts divided using a very similar system that included the FtsZ ring, the plastid-dividing/mitochondrion-dividing ring, and the dynamin ring.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algal Proteins / genetics*
  • Algal Proteins / metabolism
  • Amino Acid Sequence
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Cell Division / genetics
  • Cell Division / physiology
  • Chloroplasts / metabolism*
  • Chloroplasts / ultrastructure
  • Cytosol / metabolism
  • Dynamin II / genetics*
  • Dynamin II / metabolism
  • Dynamins / genetics*
  • Dynamins / metabolism
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism
  • Immunohistochemistry
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Rhodophyta / genetics
  • Rhodophyta / metabolism
  • Rhodophyta / ultrastructure
  • Sequence Homology, Amino Acid

Substances

  • Algal Proteins
  • Arabidopsis Proteins
  • FTSZ protein, Arabidopsis
  • Plant Proteins
  • GTP-Binding Proteins
  • Dynamin II
  • Dynamins

Associated data

  • GENBANK/AB094119