The level of MEF but not ELF-1 correlates with FAB subtype of acute myeloid leukemia and is low in good prognosis cases

Takuya Fukushima; Yasushi Miyazaki; Hideki Tsushima; Chizuko Tsutsumi; Jun Taguchi; Shinichiro Yoshida; Kazutaka Kuriyama; David Scadden; Stephen Nimer; Masao Tomonaga

doi:10.1016/s0145-2126(02)00214-x

The level of MEF but not ELF-1 correlates with FAB subtype of acute myeloid leukemia and is low in good prognosis cases

Leuk Res. 2003 May;27(5):387-92. doi: 10.1016/s0145-2126(02)00214-x.

Authors

Takuya Fukushima¹, Yasushi Miyazaki, Hideki Tsushima, Chizuko Tsutsumi, Jun Taguchi, Shinichiro Yoshida, Kazutaka Kuriyama, David Scadden, Stephen Nimer, Masao Tomonaga

Affiliation

¹ Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, Japan.

PMID: 12620289
DOI: 10.1016/s0145-2126(02)00214-x

Abstract

ETS proteins (such as PU.1, Fli-1 and ETS-1) have been shown to play important roles in normal and abnormal hematopoiesis. We examined the expression of the ELF subfamily of ETS genes (ELF-1, MEF and NERF) in acute myeloid leukemia (AML) cells using Northern blot analysis. ELF-1 and MEF were expressed in all samples, whereas NERF was not. The relative expression (RE) of MEF, but not ELF-1, was significantly lower (P<0.0001) in AML with t(8;21) and t(15;17) compared with AML with normal karyotype. The pattern of MEF expression was not uniform among cells with CD34(+)/CD33(+). It is suggested that the low RE of MEF might be part of a gene expression profile characterizing AML with a good prognosis.

Publication types

Comparative Study

MeSH terms

Acute Disease
Adult
Aged
Biomarkers, Tumor / analysis*
Biomarkers, Tumor / biosynthesis
Biomarkers, Tumor / genetics
Chromosomes, Human, Pair 15 / ultrastructure
Chromosomes, Human, Pair 17 / ultrastructure
Chromosomes, Human, Pair 21 / ultrastructure
Chromosomes, Human, Pair 8 / ultrastructure
DNA-Binding Proteins / analysis*
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics
Female
Gene Expression Regulation, Leukemic
Hematopoietic Stem Cells / chemistry
Humans
Karyotyping
Leukemia, Myeloid / classification
Leukemia, Myeloid / genetics
Leukemia, Myeloid / metabolism*
Male
Middle Aged
Prognosis
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
RNA, Neoplasm / analysis
RNA, Neoplasm / biosynthesis
Transcription Factors / analysis*
Transcription Factors / biosynthesis
Transcription Factors / genetics
Translocation, Genetic

Substances

Biomarkers, Tumor
DNA-Binding Proteins
ELF4 protein, human
RNA, Messenger
RNA, Neoplasm
Transcription Factors
ELF2 protein, human