Abstract
Immunization with the whole cell pertussis vaccine (Pw), but not the acellular pertussis vaccine (Pa), is associated with a number of neurological side effects. Previously, we have demonstrated a role for interleukin-1beta (IL-1beta) in Pw reactogenicity. Here we report that parenteral Pw administration resulted in a concomitant increase IL-1 type I receptor (IL-1RI) mRNA and a decrease in IL-1 type II receptor (IL-1RII) mRNA expression in the murine hypothalamus. These Pw-induced changes were accompanied by an increase in caspase-1 and interleukin-1beta (IL-1beta), and were associated with increased activity of the stress-activated kinase, p38. In contrast, immunization with Pa failed to activate pro-inflammatory IL-1 responses but resulted in increased IL-1 receptor antagonist (IL-1ra) production. These results suggest that the neurological effects of Pw are associated with central activation of IL-1beta and IL-1-associated signalling components.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caspase 1 / drug effects
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Caspase 1 / metabolism
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Diphtheria-Tetanus-acellular Pertussis Vaccines / pharmacology
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Female
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Hypothalamus / drug effects*
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Hypothalamus / immunology
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Hypothalamus / metabolism
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Interleukin 1 Receptor Antagonist Protein
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Interleukin-1 / metabolism*
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JNK Mitogen-Activated Protein Kinases
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Mice
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Mitogen-Activated Protein Kinases / drug effects
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Mitogen-Activated Protein Kinases / metabolism
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Pertussis Vaccine / adverse effects*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Receptors, Interleukin-1 / drug effects
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Receptors, Interleukin-1 / genetics
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Receptors, Interleukin-1 / metabolism*
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Receptors, Interleukin-1 Type I
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Receptors, Interleukin-1 Type II
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Sialoglycoproteins / drug effects
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Sialoglycoproteins / metabolism
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Signal Transduction / drug effects
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Signal Transduction / immunology
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p38 Mitogen-Activated Protein Kinases
Substances
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Diphtheria-Tetanus-acellular Pertussis Vaccines
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Il1rn protein, mouse
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Interleukin 1 Receptor Antagonist Protein
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Interleukin-1
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Pertussis Vaccine
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RNA, Messenger
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Receptors, Interleukin-1
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Receptors, Interleukin-1 Type I
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Receptors, Interleukin-1 Type II
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Sialoglycoproteins
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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Caspase 1