Jatrophane diterpenes from the latex of Euphorbia obtusifolia with inhibitory activity on the mammalian mitochondrial respiratory chain

Planta Med. 2003 Feb;69(2):177-8. doi: 10.1055/s-2003-37701.

Abstract

Seven diterpenes isolated of the latex of the Euphorbia obtusifolia were evaluated as inhibitors of the NADH oxidase activity in submitochondrial particles from bovine heart. Compound 2, 2,3,5,7,8,9,15-heptahydroxyjatropha-6(17),11-diene-14-one 8,9-diacetate 7-isobutyrate 2,3-bis(2-methylbutyrate), was the most potent inhibitor with an inhibitory concentration (IC 50 ) value of 5.1 +/- 0.2 microM. In the present study, some structure-activity trends are suggested for the inhibitory activity of these natural products on the mammalian mitochondrial respiratory chain.

Publication types

  • Letter

MeSH terms

  • Animals
  • Cattle
  • Diterpenes / administration & dosage
  • Diterpenes / pharmacology*
  • Diterpenes / therapeutic use
  • Dose-Response Relationship, Drug
  • Euphorbia*
  • Humans
  • Inhibitory Concentration 50
  • Latex / chemistry
  • Mitochondria, Heart / drug effects*
  • Mitochondria, Heart / enzymology
  • Multienzyme Complexes / antagonists & inhibitors*
  • Multienzyme Complexes / metabolism
  • NADH, NADPH Oxidoreductases / antagonists & inhibitors*
  • NADH, NADPH Oxidoreductases / metabolism
  • Oxygen Consumption / drug effects
  • Phytotherapy*
  • Structure-Activity Relationship

Substances

  • Diterpenes
  • Latex
  • Multienzyme Complexes
  • NADH oxidase
  • NADH, NADPH Oxidoreductases