Abstract
Covalent linkage of immunostimulatory CpG DNA to OVA results in CpG DNA-aided cross-presentation of OVA by dendritic cells (DCs). In vivo, cross-presentation is conditional for cross-priming of OVA-specific CD8 T cells. In this study, we investigated the involvement of the CpG DNA receptor Toll-like receptor (TLR)9 in CpG DNA-aided cross-presentation and cross-priming. Although CpG DNA-aided cross-presentation is not altered in TLR9-deficient cells, TLR9 is required for maturation of APC allowing cross-priming, as resulting in CTL function. These findings imply that TLR9 does not trigger endocytosis of CpG-OVA conjugates, but activates DCs downstream of endocytosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / metabolism
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Adjuvants, Immunologic / physiology*
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Animals
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Antigen Presentation*
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / metabolism
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CD8-Positive T-Lymphocytes / immunology*
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Cell Line
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Cells, Cultured
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CpG Islands / immunology
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DNA / immunology*
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DNA / metabolism
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / deficiency
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Endocytosis / immunology
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Immunization
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Immunoconjugates / immunology*
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Immunoconjugates / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Oligodeoxyribonucleotides / immunology*
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Oligodeoxyribonucleotides / metabolism
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Ovalbumin / immunology*
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Ovalbumin / metabolism
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Receptors, Cell Surface / biosynthesis*
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Receptors, Cell Surface / deficiency
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism
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Toll-Like Receptor 9
Substances
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Adjuvants, Immunologic
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CPG-oligonucleotide
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DNA-Binding Proteins
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Immunoconjugates
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Oligodeoxyribonucleotides
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Receptors, Cell Surface
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Tlr9 protein, mouse
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Toll-Like Receptor 9
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Ovalbumin
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DNA