Effect of endothelin-1 (1-31) on the renal resistance vessels

J Med Invest. 2003 Feb;50(1-2):87-94.

Abstract

Human chymase produces not only angiotensin II but also endothelin(ET)-1(1-31). We previously reported that ET-1(1-31) had several biological activities in vascular smooth muscle cells. In this study, we investigated the vasoconstrictor effect of ET-1(1-31) on the renal resistance vessels using in vitro microperfused rabbit afferent and efferent arterioles. ET-1(1-31) decreased the lumen diameter of the afferent and efferent arterioles dose-dependently. ET-1(1-31)-induced afferent arteriolar vasoconstriction was not affected by phosphoramidon, an ET converting enzyme inhibitor. ET-1(1-31)-induced renal arteriolar vasoconstriction was inhibited by BQ123, an ETA receptor inhibitor, but not by BQ788, an ETB receptor inhibitor. These results suggest that ET-1(1-31)-induced renal arteriolar vasoconstriction may be mediated by ETA-like receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arterioles / drug effects*
  • Arterioles / ultrastructure
  • Aspartic Acid Endopeptidases / antagonists & inhibitors
  • Dose-Response Relationship, Drug
  • Endothelin-1 / analogs & derivatives
  • Endothelin-1 / chemical synthesis
  • Endothelin-1 / pharmacology*
  • Endothelin-Converting Enzymes
  • Enzyme Inhibitors / pharmacology
  • Glycopeptides / pharmacology
  • Humans
  • Kidney / blood supply*
  • Male
  • Metalloendopeptidases
  • Muscle, Smooth, Vascular / drug effects*
  • Oligopeptides / pharmacology
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / pharmacology*
  • Peptides, Cyclic / pharmacology
  • Perfusion
  • Piperidines / pharmacology
  • Rabbits
  • Receptor, Endothelin A
  • Receptors, Endothelin / drug effects
  • Receptors, Endothelin / physiology
  • Vascular Resistance / drug effects
  • Vasoconstriction / drug effects*
  • Vasoconstrictor Agents / pharmacology*

Substances

  • Endothelin-1
  • Enzyme Inhibitors
  • Glycopeptides
  • Oligopeptides
  • Peptide Fragments
  • Peptides, Cyclic
  • Piperidines
  • Receptor, Endothelin A
  • Receptors, Endothelin
  • Vasoconstrictor Agents
  • endothelin-1 (1-31)
  • BQ 788
  • Aspartic Acid Endopeptidases
  • Metalloendopeptidases
  • Endothelin-Converting Enzymes
  • cyclo(Trp-Asp-Pro-Val-Leu)
  • phosphoramidon