Ets, Ap-1 and GATA factor families regulate the utrophin B promoter: potential regulatory mechanisms for endothelial-specific expression

Kelly J Perkins; Kay E Davies

doi:10.1016/s0014-5793(03)00175-3

Ets, Ap-1 and GATA factor families regulate the utrophin B promoter: potential regulatory mechanisms for endothelial-specific expression

FEBS Lett. 2003 Mar 13;538(1-3):168-72. doi: 10.1016/s0014-5793(03)00175-3.

Authors

Kelly J Perkins¹, Kay E Davies

Affiliation

¹ Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

PMID: 12633873
DOI: 10.1016/s0014-5793(03)00175-3

Abstract

Duchenne muscular dystrophy is caused by dystrophin deficiency, which can be prevented in the mdx mouse model by over-expression of an autosomal homologue, utrophin. Utrophin has two characterised full-length promoters, A and B. No data are available on the transcriptional regulation of B utrophin, which has been recently localised to the endothelium. Similar to characterised endothelial promoters, Ets and Ap-1 individually trans-activate the human B core promoter. Synergistic activation by GATA-2 and c-jun to the order of 20-fold was observed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
Cytoskeletal Proteins / genetics*
Electrophoretic Mobility Shift Assay
Endothelium / metabolism
Gene Expression Regulation / drug effects*
Humans
Membrane Proteins / genetics*
Mice
Molecular Sequence Data
Promoter Regions, Genetic
Sequence Homology, Nucleic Acid
Tetradecanoylphorbol Acetate / pharmacology
Transcription Factors / physiology*
Transcriptional Activation / drug effects
Utrophin

Substances

Cytoskeletal Proteins
Membrane Proteins
Transcription Factors
Utrn protein, mouse
Utrophin
Tetradecanoylphorbol Acetate