Abstract
In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Czeta, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Czeta and PP2Calpha was 22%. On Northern blot analysis PP2Czeta was found to be expressed specifically in the testicular germ cells. PP2Czeta expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Czeta exhibits its specific role through its SUMO-induced recruitment to UBC9.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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COS Cells
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DNA, Complementary
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Escherichia coli / genetics
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Isoenzymes / chemistry
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Isoenzymes / genetics
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Isoenzymes / metabolism*
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Ligases / metabolism*
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Molecular Sequence Data
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Phosphoprotein Phosphatases / chemistry
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism*
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Protein Binding
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Protein Phosphatase 2C
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Ubiquitin-Conjugating Enzymes*
Substances
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DNA, Complementary
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Isoenzymes
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Recombinant Proteins
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Ubiquitin-Conjugating Enzymes
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Phosphoprotein Phosphatases
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Protein Phosphatase 2C
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Ligases
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ubiquitin-conjugating enzyme UBC9