Abstract
We investigated the anti-proliferative effects of luteolin and apigenin, isolated from Ixeris sonchifolia Hance, on HepG2 human hepatocellular carcinoma cells. In MTT assay luteolin showed more efficient anti-proliferative effects on cells than apigenin did. According to propidium iodide staining and flow cytometry studies, we postulated that these effects might be a result of cell cyde arrest. Hence we examined the changes of protein expressions related to cell cycle arrest. Western blotting data demonstrated that the down-regulated expression of CDK4 was correlated to the increase of p53 and CDK inhibitor p21(WAF1/CIP1) protein. These data suggest that luteolin may have potential as an anti-cancer agent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Apigenin
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Asteraceae / chemistry*
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Blotting, Western
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Carcinoma, Hepatocellular / metabolism*
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Carcinoma, Hepatocellular / pathology
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Cell Division / drug effects
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclin-Dependent Kinases / biosynthesis
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Cyclins / biosynthesis
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Down-Regulation
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Drug Screening Assays, Antitumor
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology
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Flow Cytometry
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Humans
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Luteolin
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Plant Roots / chemistry
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Proto-Oncogene Proteins*
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / biosynthesis
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Up-Regulation
Substances
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Antineoplastic Agents, Phytogenic
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CDKN1A protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Flavonoids
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Proto-Oncogene Proteins
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Tumor Suppressor Protein p53
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Apigenin
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CDK4 protein, human
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases
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Luteolin